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neb restriction enzyme mix  (New England Biolabs)


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    New England Biolabs neb restriction enzyme mix
    Neb Restriction Enzyme Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1290 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/neb+restriction+enzyme+mix/MboI/bio_rxiv__2025__11__03__686253-213-18-18
    Average 98 stars, based on 1290 article reviews
    neb restriction enzyme mix - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Purification:

    Article Title: R-loops orchestrate the maternal-to-zygotic transition by harnessing RNA polymerase II pause release
    Article Snippet: .. Genomic DNA was purified from cells or embryos using a genomic DNA extraction kit and then incubated with NEB restriction enzyme mix (Alu I, Mbo I, Mse I, Dde I), Nuclease P1, T5 exonuclease, and Lambda exonuclease at 37°C for 1 h. As controls, RNase H (NEB, M0297S) was added during the incubation stage. .. 0.2 μL of Proteinase K (Thermo Scientific, EO0491) was added before incubation at 55°C for 30 min and 70°C for 30 min. 0.3 μL 1 M Tris-HCl (pH 8.0), 0.15 μL 1M MgCl 2 , 1.5 μL PEG200, 4.8 μL 50% PEG8000 and 0.5 μL transposase (Vazyme, TD503) were added and incubated at 55°C for 10 min.

    DNA Extraction:

    Article Title: R-loops orchestrate the maternal-to-zygotic transition by harnessing RNA polymerase II pause release
    Article Snippet: .. Genomic DNA was purified from cells or embryos using a genomic DNA extraction kit and then incubated with NEB restriction enzyme mix (Alu I, Mbo I, Mse I, Dde I), Nuclease P1, T5 exonuclease, and Lambda exonuclease at 37°C for 1 h. As controls, RNase H (NEB, M0297S) was added during the incubation stage. .. 0.2 μL of Proteinase K (Thermo Scientific, EO0491) was added before incubation at 55°C for 30 min and 70°C for 30 min. 0.3 μL 1 M Tris-HCl (pH 8.0), 0.15 μL 1M MgCl 2 , 1.5 μL PEG200, 4.8 μL 50% PEG8000 and 0.5 μL transposase (Vazyme, TD503) were added and incubated at 55°C for 10 min.

    Incubation:

    Article Title: R-loops orchestrate the maternal-to-zygotic transition by harnessing RNA polymerase II pause release
    Article Snippet: .. Genomic DNA was purified from cells or embryos using a genomic DNA extraction kit and then incubated with NEB restriction enzyme mix (Alu I, Mbo I, Mse I, Dde I), Nuclease P1, T5 exonuclease, and Lambda exonuclease at 37°C for 1 h. As controls, RNase H (NEB, M0297S) was added during the incubation stage. .. 0.2 μL of Proteinase K (Thermo Scientific, EO0491) was added before incubation at 55°C for 30 min and 70°C for 30 min. 0.3 μL 1 M Tris-HCl (pH 8.0), 0.15 μL 1M MgCl 2 , 1.5 μL PEG200, 4.8 μL 50% PEG8000 and 0.5 μL transposase (Vazyme, TD503) were added and incubated at 55°C for 10 min.

    Article Title: Capturing R-loops at base-pair resolution unveils clustered R-loops regulate gene expression in a number-dependent manner
    Article Snippet: .. 1 µg DNA was incubated with NEB restriction enzyme mix (Alu I, Mbo I, Mse I, Dde I), Nuclease P1, T5 exonuclease, Lambda exonuclease at 37°C for 2 hr. .. As controls, RNase H (NEB, M0297S) was added during the incubation stage.




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